Investigation of the changes in some elements that play an active role in MS disease
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Objective: In this study, we examined the role of several metals which extracted from the hair of patients who affected by multiple sclerosis (MS). The ICP-MS measurements were performed using an Agilent 7500 series. With this procedure, all elements can be simultaneously quantified.
Material and Methods: All studies were carried out at Kayseri City Hospital between August 9, 2021, and October 11, 2021. We measured five elements from hair in association with the clinical course in 40 male and female MS subjects, as well as 40 healthy volunteers, using 1 cm hair sections. Using a novel microwave digestion method coupled with ICP-MS, we were able to analyze large numbers of samples per day consisting of 0.5 g of hair in 5 mL of nitric acid. Following digestion, the aliquots were examined to determine the quantities of Hg, Pb, Cu, Cd, and Zn.
Results: The quantities recovered for all elements were in an acceptable certified value range of (97.60–99.49%). Cu, Zn, and Hg levels examined were higher in the patient group, and Cd and Pb were higher in the control group. In our patients, a positive correlation was found between age, Hg, Cd, Pb values, and the disability score of multiple sclerosis [EDSS], while there was a negative correlation between Zn and Cu values and EDSS. Additionally, female MS patients showed a higher average hair content than males for the elements studied. Both Cd and Hg levels in patients were increased compared to the controls. Lead content in hair is steadily increasing in individuals of two different age groups. Lead content in hair showed an increased trend with age in the 21-35 year and 36-60 age groups.
Conclusion: Our study shows some important changes and relationships with Pb. Also, it has been determined that the Pb element increases with age, and the Cd and Hg levels were noticeably different in the patient and healthy groups, and Cu and Zn values change in parallel. The study can be a guide for diagnosis and treatment of the MS.
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