Examination the effects of chestnut and Manuka Honey for wound healing on mice experimental model

Main Article Content

Özcan Budak
Hüseyin Çakıroğlu

Abstract

Objective: In this study, the aim is to examine the effects of Chestnut and Manuka honey on wound healing in order to investigate the effectiveness of cost-effective alternative to current approaches in terms of wound care and treatment.


Material and Methods: In this study, we used 30 healthy male Balbc mice weighing 18-24 g. We randomly divided the rats into three groups. A control group, a group treated with only Chestnut honey, a group treated with Manuka honey cream. After the wounds were formed in groups, the tissue samples were gathered on the seventh and fourteenth days. Then these samples were examined histologically and immunohistochemically.


Results: When the study results were evaluated, statistically significant differences were seen between histological and immune-histochemical findings in wound tissue preparations. On the seventh day, tissue samples showed re-epithelialization (P=0,002), granulation cell density (P=0,003) and angiogenesis (P= 0,003). In the fourteenth day tissue samples, we found epithelialization (P=0,001), granulation cell density (P=0,002) and angiogenesis (P=0,001). In the tissue samples in the seventh and fourteenth days between the groups, we found immino-histochemically, Ki-67 and EGF dyeing percentages as P= 0,004 and P=0,003 respectively.


Conclusion: We think chestnut honey may contribute to a shorter wound healing process.

Downloads

Download data is not yet available.

Article Details

How to Cite
Budak, Özcan, & Çakıroğlu, H. (2022). Examination the effects of chestnut and Manuka Honey for wound healing on mice experimental model. Medical Science and Discovery, 9(3), 170–174. https://doi.org/10.36472/msd.v9i3.700
Section
Research Article
Received 2022-03-02
Accepted 2022-03-14
Published 2022-03-26

References

Drosou A, Falabella A, Kirsner R. Antiseptics on Wounds: An Area of Controversy. Wounds. 2003;15:149-66.

Vural F, Savci A. Yara Bakımında Yeni Uygulamalar New Practices in Wound Care. Turkiye Klinikleri Journal of Nursing Sciences. 2017;3:224-32.

Mendez-Eastman S. Wound dressing categories. Plastic Surgical Nursing. 2005;25(2):95-9. DOI: https://doi.org/10.1097/00006527-200504000-00008

Yaghoobi R, Kazerouni A, Kazerouni O. Evidence for Clinical Use of Honey in Wound Healing as an Anti-bacterial, Anti-inflammatory Anti-oxidant and Anti-viral Agent: A Review. Jundishapur journal of natural pharmaceutical products. 2013;8(3):100-4. DOI: https://doi.org/10.17795/jjnpp-9487

Iftikhar F, Arshad M, Rasheed F, Amraiz D, Anwar P, Gulfraz M. Effects of acacia honey on wound healing in various rat models. Phytotherapy research : PTR. 2010;24(4):583-6. DOI: https://doi.org/10.1002/ptr.2990

Kamaratos AV, Tzirogiannis KN, Iraklianou SA, Panoutsopoulos GI, Kanellos IE, Melidonis AI. Manuka honey-impregnated dressings in the treatment of neuropathic diabetic foot ulcers. International wound journal. 2014;11(3):259-63. DOI: https://doi.org/10.1111/j.1742-481X.2012.01082.x

Mayer A, Slezak V, Takac P, Olejnik J, Majtan J. Treatment of non-healing leg ulcers with honeydew honey. Journal of tissue viability. 2014;23(3):94-7. DOI: https://doi.org/10.1016/j.jtv.2014.08.001

Stadler I, Zhang RY, Oskoui P, Whittaker MS, Lanzafame RJ. Development of a simple, noninvasive, clinically relevant model of pressure ulcers in the mouse. Journal of investigative surgery : the official journal of the Academy of Surgical Research. 2004;17(4):221-7. DOI: https://doi.org/10.1080/08941930490472046

Ulloa-Padilla JP, Ghassibi MP, Dubovy SR, Kerr DA. Clinicopathologic Correlation of Kaposi Sarcoma Involving the Ocular Adnexa: Immunophenotyping of Diagnostic and Therapeutic Targets. Ophthalmic plastic and reconstructive surgery. 2020;36(2):185-90. DOI: https://doi.org/10.1097/IOP.0000000000001506

Reid RR, Sull AC, Mogford JE, Roy N, Mustoe TA. A novel murine model of cyclical cutaneous ischemia-reperfusion injury. The Journal of surgical research. 2004;116(1):172-80. DOI: https://doi.org/10.1016/S0022-4804(03)00227-0

Al-Mamary M, Al-Meeri A, Al-Habori M. Antioxidant activities and total phenolic of different types of honey. Nutrition Research - NUTR RES. 2002;22:1041-7. DOI: https://doi.org/10.1016/S0271-5317(02)00406-2

Panchatcharam M, Miriyala S, Gayathri VS, Suguna L. Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species. Molecular and cellular biochemistry. 2006;290(1-2):87-96. DOI: https://doi.org/10.1007/s11010-006-9170-2

Akershoek JJ, Brouwer KM, Vlig M, Boekema B, Beelen RHJ, Middelkoop E, et al. Differential effects of Losartan and Atorvastatin in partial and full thickness burn wounds. PloS one. 2017;12(6):e0179350. DOI: https://doi.org/10.1371/journal.pone.0179350

Enoch S, Leaper DJ. Basic science of wound healing. Surgery (Oxford). 2005;23(2):37-42. DOI: https://doi.org/10.1383/surg.23.2.37.60352

Czubryt MP. Common threads in cardiac fibrosis, infarct scar formation, and wound healing. Fibrogenesis & Tissue Repair. 2012;5(1):19. DOI: https://doi.org/10.1186/1755-1536-5-19

Mahdavian Delavary B, van der Veer WM, van Egmond M, Niessen FB, Beelen RH. Macrophages in skin injury and repair. Immunobiology. 2011;216(7):753-62. DOI: https://doi.org/10.1016/j.imbio.2011.01.001

Haryanto H, Urai T, Mukai K, Gontijo Filho PP, Suriadi S, Sugama J, et al. Effectiveness of indonesian honey on the acceleration of cutaneous wound healing: an experimental study in mice. Wounds. 2012;24(4):110-9.

Nisbet HO, Nisbet C, Yarim M, Guler A, Ozak A. Effects of three types of honey on cutaneous wound healing. Wounds: a Compendium of Clinical Research and Practice. 2010 Nov 1;22(11):275-83.

Profyris C, Tziotzios C, Do Vale I. Cutaneous scarring: Pathophysiology, molecular mechanisms, and scar reduction therapeutics Part I. The molecular basis of scar formation. Journal of the American Academy of Dermatology. 2012;66(1):1-10; quiz 1-2. DOI: https://doi.org/10.1016/j.jaad.2011.05.055

Werner S, Grose R. Regulation of wound healing by growth factors and cytokines. Physiological reviews. 2003;83(3):835-70. DOI: https://doi.org/10.1152/physrev.2003.83.3.835

Mukai K, Koike M, Nakamura S, Kawaguchi Y, Katagiri F, Nojiri S, et al. Evaluation of the effects of a combination of Japanese honey and hydrocolloid dressing on cutaneous wound healing in male mice. Evidence-based complementary and alternative medicine : eCAM. 2015;2015:910605. DOI: https://doi.org/10.1155/2015/910605

Park J-S, An S-J, Jeong S-I, Gwon H-J, Lim Y-M, Nho Y-C. Chestnut Honey Impregnated Carboxymethyl Cellulose Hydrogel for Diabetic Ulcer Healing. Polymers (Basel). 2017;9(7):248. DOI: https://doi.org/10.3390/polym9070248