Study of Matrix Gla Protein G-7A and T-138C Gene Polymorphisms in Patients with Type 2 Diabetes Mellitus
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Abstract
Objective: Type 2 diabetes mellitus is a major risk factor for aortic calcifications and cardiovascular disease (CVD). Matrix Gla proteins (MGP) have a significant role in control of the process of calcification. The purpose of this study was to investigate the role of MGP G-7A and T-138C gene polymorphisms in development of aortic calcification and CVD in patients with type 2 diabetes mellitus.
Material and Methods: The study included 120 patients with type 2 diabetes mellitus and 134 control group. The MGP G-7A and T-138C gene polymorphisms were identified using polymerase chain reaction (PCR) and followed by restriction fragment length polymorphism (RFLP) methods.
Results: The G-7A genotype distribution in patients with type 2 diabetes mellitus AA=10.8%, GA=41.7% and GG=47.5% did not significantly differ from those in control group AA=15.7%, GA=48.5% and GG=35.8% (P=0.146). The T-138C genotype distribution in patients with type 2 diabetes mellitus CC=8.4%, CT=40.8% and TT=50.8% were also not significantly different from those in control group CC=3.7%, CT=39.6% and TT=56.7% (P=0.259). On the other hand; age, fasting blood glucose (FBG), cholesterol, high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) as expected were significantly differed between the patient-control groups (p<0.05).
Conclusion: This patient-control study show that G-7A and T-138C gene polymorphisms of MGP are not genetic risk factors for type 2 diabetes mellitus.
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